Workshop details

OriGene Biosolutions is a premier life science research and training organization dedicated to advancing bioinformatics and data analysis. Tailored solutions for your bioinformatics needs.

7-Day Workshop on Microbiome Analysis

About the Workshop


Learn how to process, analyze, and interpret microbiome sequencing data, including 16S rRNA gene analysis, metagenomics, diversity metrics, and visualization techniques.

Who Can Join


Students, researchers, and professionals interested in microbial ecology, microbiome research, and bioinformatics.

Prerequisites


Basic molecular biology and microbiology knowledge. Familiarity with command-line tools is helpful but not mandatory.

Day 1: Introduction to Microbiome and Sequencing Technologies
  • Overview of microbiomes and their importance
  • Types of microbiome sequencing (16S rRNA, shotgun metagenomics)
  • Data formats and quality control
Day 2: 16S rRNA Gene Data Processing and OTU/ASV Picking
  • Sequence trimming and filtering (QIIME2, DADA2)
  • Operational Taxonomic Units (OTUs) vs Amplicon Sequence Variants (ASVs)
Day 3: Taxonomic Classification and Diversity Analysis
  • Assigning taxonomy using reference databases (Greengenes, SILVA)
  • Alpha and beta diversity metrics
Day 4: Functional Profiling and Metagenomics Overview
  • Predicting functional profiles from 16S data (PICRUSt)
  • Introduction to shotgun metagenomics analysis
Day 5: Metagenomic Assembly and Binning
  • Assembly of metagenomic reads (MEGAHIT, metaSPAdes)
  • Binning approaches to recover genomes (MetaBAT, MaxBin)
Day 6: Visualization and Statistical Analysis
  • Visualization tools (Phyloseq, MicrobiomeAnalyst)
  • Multivariate statistics and hypothesis testing
Day 7: Case Studies and Project Presentation
  • Review of recent microbiome research applications
  • Participant project presentations
  • Discussion on challenges and future directions in microbiome research