Workshop details
OriGene Biosolutions is a premier life science research and training organization dedicated to advancing bioinformatics and data analysis. Tailored solutions for your bioinformatics needs.
3-Day Workshop on In Silico Genome Editing Using CRISPR-Cas9
About the Workshop
Learn the fundamentals and practical skills to design CRISPR guide RNAs (gRNAs), analyze off-target effects, and simulate genome editing experiments using computational tools.
Who Can Join
Life science students, researchers, and biotech professionals interested in genome editing.
Prerequisites
Basic genetics and molecular biology knowledge. Familiarity with computers and basic bioinformatics is helpful.
Day 1: Introduction to CRISPR-Cas9 and Guide RNA Design
- Overview of CRISPR-Cas9 mechanism and applications
- Principles of gRNA design: PAM sequences, on-target efficiency
- Tools for gRNA design: CHOPCHOP, Benchling, CRISPOR
- Understanding off-target effects and specificity
- Computational methods and scoring systems for off-target prediction
- In silico simulation of CRISPR edits and predicting mutation outcomes
- Designing primers for validation (PCR, sequencing)