Workshop details

OriGene Biosolutions is a premier life science research and training organization dedicated to advancing bioinformatics and data analysis. Tailored solutions for your bioinformatics needs.

3-Day Workshop on Vector Construction and Cloning Strategies

About the Workshop


This workshop covers the principles and practical approaches of vector design, cloning techniques, and in silico analysis using bioinformatics tools.

Who Can Join


Students, researchers, and lab professionals in molecular biology, biotechnology, and genetic engineering.

Prerequisites


Basic knowledge of DNA structure, cloning, and molecular biology techniques.

Day 1: Fundamentals of Vectors and Cloning
  • Types of vectors: plasmids, BACs, viral vectors
  • Essential vector elements (origin of replication, selection markers, MCS)
  • Cloning strategies and restriction enzymes
Day 2: In Silico Vector Design and Primer Design
  • Designing recombinant DNA constructs using software tools
  • Primer design for cloning (restriction sites, overhangs)
Day 3: Simulation of Cloning and Validation
  • Simulating restriction digestion and ligation in silico
  • Troubleshooting cloning experiments