Workshop details
OriGene Biosolutions is a premier life science research and training organization dedicated to advancing bioinformatics and data analysis. Tailored solutions for your bioinformatics needs.
1-Day Workshop on 16S rRNA Sequence Analysis for Microbial Identification
About the Workshop
This session provides practical knowledge and hands-on experience in analyzing 16S rRNA sequences for bacterial taxonomy, phylogenetics, and community profiling.
Who Can Join
Students, researchers, and professionals in microbiology, bioinformatics, and environmental science.
Prerequisites
Basic understanding of DNA, PCR, and microbial taxonomy.
Session 1: Introduction & Sequence Retrieval
- What is 16S rRNA and why it is used for microbial identification
- Overview of variable regions (V1–V9)
- Databases: NCBI, RDP, SILVA, Greengenes
- Hands-on: Retrieve a 16S rRNA sequence from NCBI and check metadata
- Sequence quality check (FASTA/Q)
- Perform taxonomic identification using:
- NCBI BLASTn
- RDP Classifier or EzBioCloud
- Hands-on: Assign taxonomy to unknown sequences
- Optional: Multiple sequence alignment with Clustal Omega or MAFFT
- Build phylogenetic tree from aligned 16S sequences
- Tools: MEGA, PhyloT, or iTOL
- Hands-on: Visualize and interpret evolutionary relationships
- Q&A, discussion of applications in microbiome and diagnostics